Molecular Cloning and Expression Analysis of a AGAMOUS-like 66 Gene (GbAGL66) in Ginkgo biloba
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چکیده
منابع مشابه
Molecular cloning and characterization of a novel dehydrin gene from Ginkgo biloba.
A full-length cDNA encoding a dehydrin was cloned from the living fossil plant Ginkgo biloba by rapid amplification of cDNA ends (RACE). The cDNA, designated as GbDHN, was 813 bp long containing an open reading frame of 489 bp. The deduced GbDHN protein had 163 amino acid residues, which formed a 17 kDa polypeptide with a predicted isoelectric point (pI) of 5.75. GbDHN had an S-segment and a K-...
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Ginkgolides are key pharmaceutical components in Ginkgo biloba. Using the cDNA sequence of the MECP and MECT genes to design primers, we obtained the promoters of these genes from Ginkgo genomic DNA using the genome walking method. The two promoters were 744 and 982 bp in length, respectively. The cis-elements of the GbMECPs and GbMECT promoters were predicted and analyzed using the plant cis-a...
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2C-methyl-D-erythritol 2, 4-cyclodiphosphate synthase (MECPS, EC: 4.6.1.12) is the fifth enzyme of the non-mevalonate terpenoid pathway for isopentenyl diphosphate biosynthesis and is involved in the methylerythritol phosphate (MEP) pathway for ginkgolide biosynthesis. The full-length mecps cDNA sequence (designated as Gbmecps) was cloned and characterized for the first time from gymnosperm pla...
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The WD-repeat proteins are found in eukaryotes and play an important role in the regulation of a wide variety of cellular functions including signal transduction, pre-mRNA processing and cytoskeleton assembly. In this study, a cDNA encoding a WD repeat-containing protein 13 (GbWDR13) was cloned by PCR from Ginkgo biloba. The length of GbWDR13 gene is 1500 bp, encoding 496 amino acids. The predi...
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Plants are naturally exposed to frequent changes in environmental conditions such as salt stress affecting their growth and development, and consequently a considerable reduction in total productivity. Monodehydroascorbate reductase (MDHAR), a key enzyme in ascorbate-glutathione cycle, serves as an important antioxidative enzyme in scavenging of reactive oxygen spices (ROS). In this study, ...
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ژورنال
عنوان ژورنال: Biotechnology(Faisalabad)
سال: 2017
ISSN: 1682-296X
DOI: 10.3923/biotech.2017.100.107